anti cypa primary antibody  (Boster Bio)

Journal: Frontiers in Physiology

Article Title: Extracellular cyclophilins A and C induce dysfunction of pancreatic microendothelial cells

doi: 10.3389/fphys.2022.980232

Figure Lengend Snippet: Primer sequences used in qPCR. CypA : cyclophilin A; CypB : cyclophilin B; CypC : cyclophilin C; CypD : cyclophilin D.

Article Snippet: Anti-CypA primary antibody and anti-CypB antibody were purchased from Elabscience (Madrid, Spain).

Techniques: Sequencing

Journal: Frontiers in Physiology

Article Title: Extracellular cyclophilins A and C induce dysfunction of pancreatic microendothelial cells

doi: 10.3389/fphys.2022.980232

Figure Lengend Snippet: Determination of eCyps effects on intracellular expression of CypA, CypB, CypC and CypD. (A) iCypA levels after eCypA and eCypB addition. (B) Expression of iCypA when endothelial cells were treated with eCypC. (C) iCypB levels after treatment with eCypA and eCypB. (D) Effect of eCypC on intracellular expression of CypB. (E) iCypC expression levels after eCypA and eCypB addition. (F) eCypC effects on its intracellular levels. (G) Expression of iCypD in presence of eCypA and eCypB. (H) Levels of iCypD after treatment with eCypC. Protein band expression was normalized by actin levels. The quantified bands are indicated by their molecular weight. Data are mean ± SEM of three independent experiments performed by duplicate and expressed as percentage of control cells. Statistical significance evaluated by one way ANOVA and Tukey’s tests (* p < 0.05, ** p < 0.01, *** p < 0.001, compared to control cells).

Article Snippet: Anti-CypA primary antibody and anti-CypB antibody were purchased from Elabscience (Madrid, Spain).

Techniques: Expressing, Molecular Weight, Control

Journal: Frontiers in Physiology

Article Title: Extracellular cyclophilins A and C induce dysfunction of pancreatic microendothelial cells

doi: 10.3389/fphys.2022.980232

Figure Lengend Snippet: Gene expression of Cyps in presence of eCyps A, B and C. Relative gene expression of (A) CypA , (B) CypB , (C) CypC , and (D) CypD after addition of eCyps A, B and C at 0.5 μg/ml for 24 h. CsA at 0.2 μM was added 1 h before treatment and LPS (1 μg/ml) was used as positive control. Relative gene expression was calculated with ΔΔCt method. Control cells were used as calibrator and GAPDH as internal normalization control. Data are expressed as mean ± SEM of three independent replicates performed by triplicate. Statistical significance evaluated by one way ANOVA and Tukey’s tests (* p < 0.05, ** p < 0.01, *** p < 0.001, compared to control cells; # p < 0.05, ## p < 0.01 pairwise comparison as indicated).

Article Snippet: Anti-CypA primary antibody and anti-CypB antibody were purchased from Elabscience (Madrid, Spain).

Techniques: Gene Expression, Positive Control, Control, Comparison

Journal: Frontiers in Physiology

Article Title: Extracellular cyclophilins A and C induce dysfunction of pancreatic microendothelial cells

doi: 10.3389/fphys.2022.980232

Figure Lengend Snippet: Summary of eCyps effects in MS1 cells.

Article Snippet: Anti-CypA primary antibody and anti-CypB antibody were purchased from Elabscience (Madrid, Spain).

Techniques: Expressing, Gene Expression

Journal: International Journal of Molecular Sciences

Article Title: Cyclophilin A in Arrhythmogenic Cardiomyopathy Cardiac Remodeling

doi: 10.3390/ijms20102403

Figure Lengend Snippet: Immunofluorescence staining of ACM RV section ( a ). Particular of a preadipocyte, positive for CD29 and perilipin 1 (PLIN1), expressing cyclophilin A (CyPA) ( b ). The image of merged signals is also shown.

Article Snippet: After washing in PBS, slides were blocked for 30 min in 10% goat serum-PBS (Sigma Aldrich, St. Louis, MO, USA) and incubated with specific primary antibodies against CyPA (1:100, sc-133494; Santa Cruz Biotechnology, Santa Cruz, CA, USA), PLIN1 (1:100, BP5015; OriGene, Herford, Germany) and CD29 (1:200, NCL-CD29; Leica, Wetzlar, Germany) in 2% goat serum-PBS O/N at 4 °C.

Techniques: Immunofluorescence, Staining, Expressing